Trypan blue induced teratogenesis of rat embryos cultivated in vitro.
نویسنده
چکیده
Trypan blue is known to produce embryonic abnormalities in a wide variety of animals, including rats (Gillman, Gilbert, Gillman & Spence, 1948), mice (Waddington & Carter, 1953; Hamburgh, 1954), amphibians (Waddington & Perry, 1956), chickens (Beaudoin & Wilson, 1958;Stephan & Sutter, 1961), rabbits (Ferm, 1956), and hamsters (Ferm, 1958). Studies on the teratogenic action of this dye have also been made in culture (Mulherkar, 1960). Since the first observation of the teratogenic action of trypan blue in rat embryos (Gillman et al. 1948), two questions that have remained unanswered are (1) whether the dye acts directly on the embryo or via the maternal system, and (2) what determines the period during which the rat embryo is most sensitive to the dye. The following account is an extension of a preliminary report (Turbow, 1965) on the direct effect of trypan blue on the isolated rat embryo cultivated in vitro and the role of the yolk-sac placenta in determining the period of sensitivity to the teratogenic action of the dye; the possible mechanisms of the teratogenic effect of the dye have also been investigated, and the results are discussed.
منابع مشابه
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عنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 15 3 شماره
صفحات -
تاریخ انتشار 1966